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Primary Antibodies
Smad3 (Phospho-Ser204) Antibody
Smad3 (Phospho-Ser204) Antibody

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: A1004
Reactivity:Human,Mouse,Rat
Applications:WB,IHC-p,IF/ICC,ELISA
Modification:Phospho Specific
Source:Polyclonal Rabbit
Storage and Stability:-20°C/1 year

$205
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Smad3 (Phospho-Ser204) Antibody

Western blot analysis of lysates from NIH/3T3 cells treated with Serum 20% 15', using Smad3 (Phospho-Ser204) Antibody. The lane on the right is blocked with the phospho peptide.

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Product Details
Product Name
Smad3 (Phospho-Ser204) Antibody
Description
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]
Catalog No
A1004
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF/ICC, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
SMAD3; MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3
Molecular Weight (Da)
48081
Gene Name
SMAD3
Protein Name
Mothers against decapentaplegic homolog 3
Human Gene ID
4088
Human Swiss Prot No.
P84022
Immunogen
The antiserum was produced against synthesized peptide derived from human Smad3 around the phosphorylation site of Ser204. AA range:170-219
Specificity
Phospho-Smad3 (S204) Polyclonal Antibody detects endogenous levels of Smad3 protein only when phosphorylated at S204.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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