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Primary Antibodies
Smad3 (Phospho-Thr179) Antibody
Smad3 (Phospho-Thr179) Antibody

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: A1003
Reactivity:Human,Mouse,Rat
Applications:WB,IHC-p,IF(paraffin section),ELISA
Modification:Phospho Specific
Source:Polyclonal Rabbit
Storage and Stability:-20°C/1 year

$205
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Smad3 (Phospho-Thr179) Antibody

Western Blot analysis of HELA cells using Phospho-Smad3 (T179) Polyclonal Antibody

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Product Details
Product Name
Smad3 (Phospho-Thr179) Antibody
Description
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]
Catalog No
A1003
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
SMAD3; MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3
Molecular Weight (Da)
48081
Gene Name
SMAD3
Protein Name
Mothers against decapentaplegic homolog 3
Human Gene ID
4088
Human Swiss Prot No.
P84022
Immunogen
The antiserum was produced against synthesized peptide derived from human Smad3 around the phosphorylation site of Thr179. AA range:145-194
Specificity
Phospho-Smad3 (T179) Polyclonal Antibody detects endogenous levels of Smad3 protein only when phosphorylated at T179.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Publications (2)
ANRIL inhibits p15INK4b through the TGFβ1 signaling pathway in human esophageal squamous cell carcinoma

Author: Deyu Chen, Zhaoyue Zhang, Chaoming Mao, Yuepeng Zhou, Lichao Yu, Yue Yin, Shi Wu, Xiao Mou, Yan Zhu

Date of Publication : 2014-04-03

Differential Regulation of Smad3 and of the Type II Transforming Growth Factor-β Receptor in Mitosis: Implications for Signaling

Author: Tal Hirschhorn, Lior Barizilay, Nechama I. Smorodinsky, Nechama I. Smorodinsky,

Date of Publication : n/a

Primary Antibodies

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    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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