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> Products > Primary Antibodies > Smad3 Antibody

Smad3 Antibody

Overview Description
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Catalog No : B1003
  • Reactivity
    Human,Mouse,Rat
  • Applications
    IF/ICC,WB,IHC-p,ELISA
  • Modification
    Unmodified/Total
  • Source
    Polyclonal Rabbit
  • Storage and Stability
    -20°C/1 year
  • Size
    50 μl
  • Price/Quantity
    $205
  • Quantity
Add to Cart
Details
  • Product Name
    Smad3 Antibody
  • Description
    The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]
  • Catalog No
    B1003
  • Reactivity
    Human, Mouse, Rat
  • Applications
    IF/ICC, WB, IHC-p, ELISA
  • Modification
    Unmodified/Total
  • Source
    Polyclonal Rabbit
  • Dilution
    IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
  • Purification
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Concentration
    1 mg/ml
  • Storage and Stability
    -20°C/1 year
  • Other Name
    SMAD3; MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3
  • Molecular Weight (Da)
    48081
  • Gene Name
    SMAD3
  • Protein Name
    Mothers against decapentaplegic homolog 3
  • Human Gene ID
    4088
  • Human Swiss Prot No.
    P84022
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human Smad3. AA range:145-194
  • Specificity
    Smad3 Polyclonal Antibody detects endogenous levels of Smad3 protein.
  • Formulation
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Application Data
Western blot analysis of lysates from HT-29 cells, using Smad3 Antibody. The lane on the right is blocked with the synthesized peptide.
Application Data
Western Blot analysis of HT29 cells using Smad3 Polyclonal Antibody diluted at 1:500
Application Data
Western Blot analysis of various cells using Smad3 Polyclonal Antibody diluted at 1:500
Application Data
Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,Smad3 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,Smad3 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue. 1,Smad3 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,Smad3 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,Smad3 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Rat-brain tissue. 1,Smad3 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,Smad3 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,Smad3 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1,Smad3 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,Smad3 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1,Smad3 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,Smad3 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,Smad3 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunofluorescence analysis of mouse-kidney tissue. 1,Smad3 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Application Data
Immunofluorescence analysis of mouse-kidney tissue. 1,Smad3 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Application Data
Immunofluorescence analysis of rat-kidney tissue. 1,Smad3 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Application Data
Immunofluorescence analysis of rat-kidney tissue. 1,Smad3 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Application Data
Immunofluorescence analysis of rat-lung tissue. 1,Smad3 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Application Data
Immunofluorescence analysis of rat-lung tissue. 1,Smad3 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Application Data
Immunofluorescence analysis of Hela cell. 1,Smad3 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). β-actin Monoclonal Antibody(5B7)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:SA0837 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:SA0662 was diluted at 1:1000(room temperature, 50min).
Application Data
Jiang, L., Liu, B., Qi, Y. et al. Antagonistic effects of activin A and TNF-α on the activation of L929 fibroblast cells via Smad3-independent signaling. Sci Rep 10, 20623 (2020). 
Publications

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    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

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