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Primary Antibodies
Smad3 Antibody
Smad3 Antibody

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: B1003
Reactivity:Human,Mouse,Rat
Applications:IF,WB,IHC,ELISA
Modification:Unmodified/Total
Source:Polyclonal,Rabbit,IgG
Storage and Stability:Store at -20°C/1 year

$205
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Smad3 Antibody

Immunofluorescence analysis of Hela cell. 1,Smad3 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). β-actin Monoclonal Antibody(5B7)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:SA0837 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:SA0662 was diluted at 1:1000(room temperature, 50min).

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Product Details
Product Name
Smad3 Antibody
Description
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009],
Catalog No
B1003
Reactivity
Human, Mouse, Rat
Source
Polyclonal, Rabbit,IgG
Modification
Unmodified/Total
Applications
IF, WB, IHC, ELISA
Dilution
IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
Store at -20°C/1 year
Other Name
SMAD3; MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3
Molecular Weight (Da or kDa)
Gene Name
SMAD3
Protein Name
Mothers against decapentaplegic homolog 3
Human Gene ID
4088
Human Swiss Prot No.
P84022
Immunogen
The antiserum was produced against synthesized peptide derived from human Smad3. AA range:145-194
Specificity
Smad3 Polyclonal Antibody detects endogenous levels of Smad3 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Publications (5)
IL-37 suppresses hepatocellular carcinoma growth by converting pSmad3 signaling from JNK/pSmad3L/c-Myc oncogenic signaling to pSmad3C/P21 tumor-suppressive signaling. Oncotarget Oncotarget. 2016 Dec 20; 7(51): 85079–85096 WB Human SMMC-7721 cell

Author: Available in link provided

Date of Publication : Available in link provided

Profilin 2 promotes growth, metastasis, and angiogenesis of small cell lung cancer through cancer-derived exosomes. Aging-US Aging-Us. 2020 Dec 31; 12(24): 25981–25999 IHC，WB Human Small cell lung cancer (SCLC)，normal lung tissue H446 cell

Author: Available in link provided

Date of Publication : Available in link provided

CD105 overexpression mediates drug-resistance in choriocarcinoma cells through BMP9/Smad pathway. Journal of Cancer J Cancer. 2020; 11(2): 272–283 WB Human 1:2000 JEG-3 cell

Author: Available in link provided

Date of Publication : Available in link provided

Inhibitory effect of tranilast on the myofibroblast differentiation of rat mesenchymal stem cells induced by transforming growth factor‑β1 in vitro. Molecular Medicine Reports Mol Med Rep. 2018 Dec;18(6):5693-5700 WB Rat 1:500 MSCs

Author: Available in link provided

Date of Publication : Available in link provided

Antagonistic effects of activin A and TNF-α on the activation of L929 fibroblast cells via Smad3-independent signaling. Scientific Reports 2020 Nov 26 WB Mouse L929 cell

Author: Available in link provided

Date of Publication : Available in link provided

Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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