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> Products > Primary Antibodies > PERK Antibody

PERK Antibody

Overview Description
Datasheet SDS
   Kinases and Phosphatases   
Catalog No : B8168
  • Reactivity
    Human,Mouse,Rat
  • Applications
    IF/ICC,WB,IHC-p,ELISA
  • Modification
    Unmodified/Total
  • Source
    Polyclonal Rabbit
  • Storage and Stability
    -20°C/1 year
  • Size
    50 μl
  • Price/Quantity
    $205
  • Quantity
Add to Cart
Details
  • Product Name
    PERK Antibody
  • Description
    The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015]
  • Catalog No
    B8168
  • Reactivity
    Human, Mouse, Rat
  • Applications
    IF/ICC, WB, IHC-p, ELISA
  • Modification
    Unmodified/Total
  • Source
    Polyclonal Rabbit
  • Dilution
    IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
  • Purification
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Concentration
    1 mg/ml
  • Storage and Stability
    -20°C/1 year
  • Other Name
    EIF2AK3; PEK; PERK; Eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; HsPEK
  • Molecular Weight (Da)
    125147
  • Gene Name
    EIF2AK3
  • Protein Name
    Eukaryotic translation initiation factor 2-alpha kinase 3
  • Human Gene ID
    9451
  • Human Swiss Prot No.
    Q9NZJ5
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human EIF2AK3. AA range:947-996
  • Specificity
    PERK Polyclonal Antibody detects endogenous levels of PERK protein.
  • Formulation
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Application Data
Western blot analysis of lysates from MCF-7 cells, using PEK/PERK Antibody. The lane on the right is blocked with the synthesized peptide.
Application Data
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using PEK/PERK Antibody. The picture on the right is blocked with the synthesized peptide.
Application Data
Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,PERK Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,PERK Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,PERK Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,PERK Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,PERK Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1,PERK Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,PERK Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Human-Appendix tissue. 1,PERK Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1,PERK Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1,PERK Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,PERK Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1,PERK Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunofluorescence analysis of mouse-lung tissue. 1,PERK Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Application Data
Immunofluorescence analysis of mouse-lung tissue. 1,PERK Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Application Data
Immunofluorescence analysis of rat-spleen tissue. 1,PERK Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Application Data
Immunofluorescence analysis of rat-spleen tissue. 1,PERK Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Application Data
Immunofluorescence analysis of rat-lung tissue. 1,PERK Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Application Data
Immunofluorescence analysis of rat-lung tissue. 1,PERK Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Application Data
Immunofluorescence analysis of Hela cell. 1,PERK Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:SA0665 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.
Publications

Primary Antibodies

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    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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