Histone H3 (Di Methyl Lys4) (AB2191) Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M3528

Species Reactivity:Human,Mouse,Rat

Application:WB,IHC,IF,IP,ELISA

Host:Rabbit

Clonality:Monoclonal

Storage and Stability:Store at-20℃. Avoid freeze / thaw cycles.

Size:

Price/Quantity $135

Quantity:

Datasheet

SDS

Recombinant Antibody

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Histone H3 (Di Methyl Lys4) (AB2191) Rabbit mAb

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Lead Time

Antibodies 1-2 business day, ELISA kits 2-3 business day lead time

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Product Details

Product Name

Histone H3 (Di Methyl Lys4) (AB2191) Rabbit mAb

Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21. 3. [provided by RefSeq, Aug 2015],

Catalog No

M3528

Species Reactivity

Human, Mouse, Rat

Clonality

Monoclonal

Host

Rabbit

Modifcation

DiMethyl K4

Clone

AB2191

Isotype

IgG

Application

WB, IHC, IF, IP, ELISA

Application Dilutions

WB, 1:2000-1:10000 | IHC, 1:1000-1:5000 | IF, 1:200-1:1000 | IP, 0. 5μg-4μg antibody for 200μg-400μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.

Immunogen

Recombinant protein (or fragment). This information is considered to be commercially sensitive.

Specificity

The antibody detects endogenous levels of Histone H3 protein only when di-methylatedat Lys4. The antibody does not cross-react with mono-methylated or tri-methylated Histone H3 Lys4 and other di-methylated sites.

Formulation

The antibody is provided in liquid form in phosphate - buffered saline with 50% glycerol, 0.05% BSA, and 0.05% Proclin 300.

Purification

The antibody was purified by protein A affinity chromatography.

Storage and Stability

Store at-20℃. Avoid freeze / thaw cycles.

Gene Name

HIST1H3A/HIST1H3/HIST1H3C/HIST1H3D/HIST1H3E/HIST1H3F/HIST1H3G/HIST1H3H/HIST1H3I/HIST1H3J/HIST2H3A/HIST2H3C/HIST2H3D/H3F3A/H3F3B/H3F3C

Gene ID

8350; 8351; 8352; 8353; 8354; 8355; 8356; 8357; 8358; 8968; 126961; 333932; 653604; 3020; 3021; 440093

Human swiss

P68431

Protein Name

Histone H3 (Di Methyl Lys4)

Synonyms

H3K4ME2, HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, HIST1H3I, H3FF, HIST1H3J, H3FJ, Histone H3. 1, Histone H3. 1, Histone H3. 2, Histone H3/m, Histone H3/o, H3F3A, H3. 3A, H3F3, PP781, H3F3B, H3. 3B, Histone H3. 3, H3F3C, Histone H3. 3C, Histone H3. 5