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Primary Antibodies
Alpha-Fetoprotein (AFP)
Alpha-Fetoprotein (AFP)

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M2156
Reactivity:Human
Applications:WB,ELISA
Modifcation:
Source:Rabbit
Storage and Stability:Store at -20℃/ 1 year. Avoid freeze / thaw cycles.

$150
# SDS #
Recombinant Antibody
20ul Size Available
#
#

Alpha-Fetoprotein (AFP)

Western blot analysis of various lysates using Alpha-Fetoprotein (AFP) Rabbit mAb (A22053) at1:2000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.

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Product Details
Product Name
Alpha-Fetoprotein (AFP)
Description
This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatocarcinoma and with teratoma, and has prognostic value for managing advanced gastric cancer. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
Catalog No
M2156
Reactivity
Human
Applications
WB, ELISA
Application Dilutions
WB,1:1000 - 1:5000 | ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Modifcation
Source
Rabbit
Isotype
IgG
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 19-609 of human Alpha-Fetoprotein (AFP) (NP_001125.1).
Calculated MW (kDa)
69kDa
Observed MW (kDa)Gene Name
69kDaAFP
Other Name
AFPD; FETA; HPAFP; Alpha-Fetoprotein (AFP)
Gene ID
174
Human Swiss Prot
P02771
Formulation
PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Storage and Stability
Store at -20℃/ 1 year. Avoid freeze / thaw cycles.
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