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Phospho-Cdc6-S54 (AX3318) Rabbit mAb
Phospho-Cdc6-S54 (AX3318) Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M1318
Reactivity:Mouse
Applications:WB,ELISA
Modifcation:Phospho S54
Source:Rabbit
Storage and Stability:Store at -20℃/ 1 year. Avoid freeze / thaw cycles.

$150
# SDS #
Recombinant Antibody
20ul Size Available
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Phospho-Cdc6-S54 (AX3318) Rabbit mAb

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Cdc6-S54 Rabbit mAb (M1318) at 1:1000 dilution. NIH/3T3 cells were treated by Paclitaxel (1 uM) at 37℃ for 20 hours. NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (SA0002) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 5s.

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Product Details
Product Name
Phospho-Cdc6-S54 (AX3318) Rabbit mAb
Description
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins.
Catalog No
M1318
Reactivity
Mouse
Applications
WB, ELISA
Application Dilutions
WB,1:1000 - 1:6000 | ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Modifcation
Phospho S54
Source
Rabbit
Isotype
IgG
Immunogen
A synthetic phosphorylated peptide around S54 of human Cdc6 (Q99741).
Calculated MW (kDa)
63kDa
Observed MW (kDa)Gene Name
63kDaCDC6
Other Name
CDC18L; HsCDC6; MGORS5; HsCDC18; Phospho-Cdc6-S54
Gene ID
990
Human Swiss Prot
Q99741
Formulation
PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Storage and Stability
Store at -20℃/ 1 year. Avoid freeze / thaw cycles.
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Primary Antibodies

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    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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