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Phospho-CRK/CRKII-Y221 (AX3277) Rabbit mAb
Phospho-CRK/CRKII-Y221 (AX3277) Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M1277
Reactivity:Human,Mouse,Rat
Applications:WB,ELISA
Modifcation:Phospho Y221
Source:Rabbit
Storage and Stability:Store at -20℃/ 1 year. Avoid freeze / thaw cycles.

$150
# SDS #
Recombinant Antibody
20ul Size Available
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Phospho-CRK/CRKII-Y221 (AX3277) Rabbit mAb

Western blot analysis of various lysates using Phospho-CRK/CRKII-Y221 Rabbit mAb (M1277) at 1:1000 dilution. Both NIH/3T3 cells and C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (SA0002) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 10s.

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Product Details
Product Name
Phospho-CRK/CRKII-Y221 (AX3277) Rabbit mAb
Description
This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described.
Catalog No
M1277
Reactivity
Human, Mouse, Rat
Applications
WB, ELISA
Application Dilutions
WB,1:500 - 1:1000 | ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Modifcation
Phospho Y221
Source
Rabbit
Isotype
IgG
Immunogen
A synthetic phosphorylated peptide around Y221 of human CRK/CRKIICRK (P46108).
Calculated MW (kDa)
34kDa
Observed MW (kDa)Gene Name
34kDaCRK
Other Name
p38; CRKII; Phospho-CRK/CRKII-Y221
Gene ID
1398
Human Swiss Prot
P46108
Formulation
PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Storage and Stability
Store at -20℃/ 1 year. Avoid freeze / thaw cycles.
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Primary Antibodies

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