Cytokeratin 7 (AB1531) mouse mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: W0319

Species Reactivity:Human;

Application:IHC; WB; IF; ELISA

Host:Mouse

Clonality:Monoclonal

Storage and Stability:Store at-20℃. Avoid freeze / thaw cycles.

Size:

Price/Quantity $135

Quantity:

Datasheet

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Cytokeratin 7 (AB1531) mouse mAb

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Lead Time

Antibodies 1-2 business day, ELISA kits 2-3 business day lead time

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408-747-0185

Fax

408-747-0145

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Product Details

Product Name

Cytokeratin 7 (AB1531) mouse mAb

Description

CK7 is a cytokeratin with a molecular weight of 54 kDa, which mainly marks glandular epithelium and transitional epithelium. It was positive in the epithelium of breast, thyroid, ovary, endometrium and lung, but negative in the epithelium of gastrointestinal tract, colon and prostate. It is mainly used for the study of epithelial tumors. It is often used in combination with CK20 for differential diagnosis of gastrointestinal adenocarcinoma, lung adenocarcinoma and breast cancer, and combined with EMA for diagnosis of synovial sarcoma.

Catalog No

W0319

Species Reactivity

Human;

Clonality

Monoclonal

Host

Mouse

Modifcation

Unmodified

Clone

AB1531

Isotype

IgG2b, kappa

Application

IHC; WB; IF; ELISA

Application Dilutions

IHC, 1:200-1:1000 | WB, 1:500-1:2000 | IF, 1:100-1:500 | ELISA, Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.

Immunogen

A synthetic peptide corresponding to the amino acid region 350 - 469 of the human Cytokeratin 7 protein.

Specificity

The antibody can specifically recognize human CK7 protein, and shows no cross reaction with CK1, 4, 5, 6, 8, 10, 14, 17, 18, 19, 20. In western blotting of Hela, A431, A549 and Hela cell lysates, the antibody can label a 51 kDa band corresponding to CK7 in A549 and Hela cell lysates, while there is no band detected in CK7 knock out Hela cell lysate.

Formulation

The antibody is provided in liquid form in phosphate - buffered saline with 50% glycerol, 0.05% BSA, and 0.05% Proclin 300.

Purification

The antibody was purified by protein G affinity chromatography.

Storage and Stability

Store at-20℃. Avoid freeze / thaw cycles.

Gene Name

KRT7

Gene ID

3855

Human swiss

P08729

Protein Name

Cytokeratin 7

Synonyms

CK 7, CK-7, CK7, Cytokeratin 7, CytokeRatin-7, D15Wsu77e, K2C7, K2C7_HUMAN, K7, KeRatin 7, KeRatin 7, type II, KeRatin type II cytoskeletal 7, KeRatin, 55K type II cytoskeletal, KeRatin, simple epithelial, KeRatin, simple epithelial type I, K7, KeRatin, type II cytoskeletal 7, KeRatin-7, Krt2-7, KRT7, MGC11625, MGC129731, MGC3625, Sarcolectin, SCL, Type II mesothelial keRatin K7, Type-II keRatin Kb7