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Primary Antibodies
Phospho-Rpb1 CTD-T4 mAb
Phospho-Rpb1 CTD-T4 mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M2762
Reactivity:Human,Mouse,Rat
Applications:WB,ELISA
Modifcation:Phospho T4
Source:Rabbit
Storage and Stability:Store at -20℃/ 1 year. Avoid freeze / thaw cycles.

$150
# SDS #
Recombinant Antibody
20ul Size Available
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Phospho-Rpb1 CTD-T4 mAb

Western blot analysis of lysates from NIH/3T3 cells using Phospho-FAK-Y576/Y577 Rabbit mAb (AP1547) at 1:16000 dilution incubated overnight at 4℃. NIH/3T3 cells were treated by pervanadate (1 mM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3 % nonfat dry milk in TBST.| Detection: ECL Basic Kit (RM00020).| Exposure time: 10s.

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Product Details
Product Name
Phospho-Rpb1 CTD-T4 mAb
Description
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
Catalog No
M2762
Reactivity
Human, Mouse, Rat
Applications
WB, ELISA
Application Dilutions
WB,1:1000 - 1:5000 | ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Modifcation
Phospho T4
Source
Rabbit
Isotype
IgG
Immunogen
A synthetic phosphorylated peptide around T4 of human POLR2A.
Calculated MW (kDa)
217kDa
Observed MW (kDa)Gene Name
270kDaPOLR2A
Other Name
RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; NEDHIB; RpIILS; hsRPB1; hRPB220
Gene ID
5430
Human Swiss Prot
P24928
Formulation
PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Storage and Stability
Store at -20℃/ 1 year. Avoid freeze / thaw cycles.
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Primary Antibodies

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    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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