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Phospho-VASP-S239 Rabbit mAb
Phospho-VASP-S239 Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M2759
Reactivity:Human
Applications:WB,IF/ICC,ELISA
Modifcation:Phospho S239
Source:Rabbit
Storage and Stability:Store at -20℃/ 1 year. Avoid freeze / thaw cycles.

$150
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Recombinant Antibody
20ul Size Available
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Phospho-VASP-S239 Rabbit mAb

Western blot analysis of lysates from C6 cells, using Phospho-VASP-S157 Rabbit mAb (AP1510) at 1:4000 dilution. C6 cells were treated by Forskolin (30 uM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 45s.

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Product Details
Product Name
Phospho-VASP-S239 Rabbit mAb
Description
Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.
Catalog No
M2759
Reactivity
Human
Applications
WB, IF/ICC, ELISA
Application Dilutions
WB,1:1000 - 1:5000 | IF/ICC,1:50 - 1:200 | ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Modifcation
Phospho S239
Source
Rabbit
Isotype
IgG
Immunogen
A synthetic phosphorylated peptide around S239 of human VASP (NP_003361.1).
Calculated MW (kDa)
40kDa
Observed MW (kDa)Gene Name
40-50kDaVASP
Other Name
VASP; vasodilator-stimulated phosphoprotein; Phospho-VASP-S239
Gene ID
7408
Human Swiss Prot
P50552
Formulation
PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Storage and Stability
Store at -20℃/ 1 year. Avoid freeze / thaw cycles.
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Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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