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Phospho-PLC gamma 1 (PLCG1)-S1248 Rabbit mAb
Phospho-PLC gamma 1 (PLCG1)-S1248 Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M2756
Reactivity:Human,Mouse
Applications:WB,ELISA
Modifcation:Phospho S1248
Source:Rabbit
Storage and Stability:Store at -20℃/ 1 year. Avoid freeze / thaw cycles.

$150
# SDS #
Recombinant Antibody
20ul Size Available
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Phospho-PLC gamma 1 (PLCG1)-S1248 Rabbit mAb

Western blot analysis of lysates from HeLa cells using Phospho-NFKB1-S337 Rabbit mAb (AP1528) at 1:3000 dilution incubated overnight at 4℃. HeLa cells were treated by TNF-α (20 ng/ml) and Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3 % nonfat dry milk in TBST.| Detection: ECL Basic Kit (RM00020).| Exposure time: 1s.

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Product Details
Product Name
Phospho-PLC gamma 1 (PLCG1)-S1248 Rabbit mAb
Description
The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.
Catalog No
M2756
Reactivity
Human, Mouse
Applications
WB, ELISA
Application Dilutions
WB,1:1000 - 1:5000 | ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Modifcation
Phospho S1248
Source
Rabbit
Isotype
IgG
Immunogen
A synthetic phosphorylated peptide around S1248 of human PLC gamma 1 (PLCG1) (NP_877963.1).
Calculated MW (kDa)
149kDa
Observed MW (kDa)Gene Name
152kDaPLCG1
Other Name
PLC1; NCKAP3; PLC-II; PLC148; PLCgamma1
Gene ID
5335
Human Swiss Prot
P19174
Formulation
PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Storage and Stability
Store at -20℃/ 1 year. Avoid freeze / thaw cycles.
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Primary Antibodies

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    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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