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Phospho-GSK3β-Y216 + GSK3α-Y279 Rabbit mAb
Phospho-GSK3β-Y216 + GSK3α-Y279 Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M2752
Reactivity:Mouse
Applications:WB,ELISA
Modifcation:Phospho Y216/Y279
Source:Rabbit
Storage and Stability:Store at -20℃/ 1 year. Avoid freeze / thaw cycles.

$150
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Recombinant Antibody
20ul Size Available
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Phospho-GSK3β-Y216 + GSK3α-Y279 Rabbit mAb

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Smad5-S465 Rabbit mAb (AP1411) at 1:2000 dilution. NIH/3T3 cells were treated by BMP4(50ng/ml) at 37℃ for 6 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.

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Product Details
Product Name
Phospho-GSK3β-Y216 + GSK3α-Y279 Rabbit mAb
Description
The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Catalog No
M2752
Reactivity
Mouse
Applications
WB, ELISA
Application Dilutions
WB,1:1000 - 1:4000 | ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Modifcation
Phospho Y216/Y279
Source
Rabbit
Isotype
IgG
Immunogen
A synthetic phosphorylated peptide around Y216&Y279 of human GSK3β/GSK3α (NP_063937.2).
Calculated MW (kDa)
51kDa/46kDa
Observed MW (kDa)Gene Name
46kDa/51kDaGSK3A/GSK3B
Other Name
GSK3B; GSK 3 beta; GSK3A; GSK 3 alpha; Phospho-GSK3α-S21+GSK3β-S9
Gene ID
2931/2932
Human Swiss Prot
P49840/P49841
Formulation
PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Storage and Stability
Store at -20℃/ 1 year. Avoid freeze / thaw cycles.
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Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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