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Phospho-PLC gamma 2 (PLCG2)-Y759 Rabbit mAb
Phospho-PLC gamma 2 (PLCG2)-Y759 Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M2751
Reactivity:Human
Applications:WB,IF/ICC,ELISA
Modifcation:Phospho Y759
Source:Rabbit
Storage and Stability:Store at -20℃/ 1 year. Avoid freeze / thaw cycles.

$150
# SDS #
Recombinant Antibody
20ul Size Available
#
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Phospho-PLC gamma 2 (PLCG2)-Y759 Rabbit mAb

Western blot analysis of lysates from NIH/3T3 cells using Phospho-GSK3β-Y216 + GSK3α-Y279 Rabbit mAb (AP1530) at 1:1000 dilution incubated overnight at 4℃. NIH/3T3 cells were treated by λ-PP mixed solution (1μL) at 30℃ for 30 minutes. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 30 μg per lane.| Blocking buffer: 3 % nonfat dry milk in TBST.| Detection: ECL Basic Kit (RM00020).| Exposure time: 20s.

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Product Details
Product Name
Phospho-PLC gamma 2 (PLCG2)-Y759 Rabbit mAb
Description
The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3.
Catalog No
M2751
Reactivity
Human
Applications
WB, IF/ICC, ELISA
Application Dilutions
WB,1:2000 - 1:6000 | IF/ICC,1:50 - 1:200 | ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Modifcation
Phospho Y759
Source
Rabbit
Isotype
IgG
Immunogen
A synthetic phosphorylated peptide around Y759 of human PLC gamma 2 (PLCG2) (NP_002652.2).
Calculated MW (kDa)
148kDa
Observed MW (kDa)Gene Name
150kDaPLCG2
Other Name
FCAS3; APLAID; PLC-IV; PLC-gamma-2; Phospho-PLC gamma 2 (PLCG2)-Y759
Gene ID
5336
Human Swiss Prot
P16885
Formulation
PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Storage and Stability
Store at -20℃/ 1 year. Avoid freeze / thaw cycles.
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Primary Antibodies

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    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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