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Primary Antibodies
Human IgG4 (Fc) Rabbit mAb
Human IgG4 (Fc) Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M2415
Reactivity:Human
Applications:WB,IF/ICC,ELISA
Modifcation:Unmodified
Source:Rabbit
Storage and Stability:Store at -20℃/ 1 year. Avoid freeze / thaw cycles.

$150
# SDS #
Recombinant Antibody
20ul Size Available
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Human IgG4 (Fc) Rabbit mAb

Western blot analysis of various lysates, using RARG Rabbit mAb (M2416) at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (SA0002) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 20s.

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Product Details
Product Name
Human IgG4 (Fc) Rabbit mAb
Description
As a monomeric immunoglobulin that is predominately involved in the secondary antibody response and the only isotype that can pass through the human placenta, Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells, and constitutes 75% of serum immunoglobulins in humans. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as the antibody-dependent cell-mediated cytotoxicity (ADCC). IgG tetramer contains two heavy chains (50 kDa ) and two light chains (25 kDa) linked by disulfide bonds, that is the two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment).
Catalog No
M2415
Reactivity
Human
Applications
WB, IF/ICC, ELISA
Application Dilutions
WB,1:1000 - 1:5000 | IF/ICC,1:100 - 1:500 | ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Modifcation
Unmodified
Source
Rabbit
Isotype
IgG
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 96-115 of Human IgG4 (Fc). (P01861).
Calculated MW (kDa)
36kDa
Observed MW (kDa)Gene Name
55kDaIGHG4
Other Name
IGHG4; Ig gamma-4 chain C region; Human IgG4 (Fc)
Gene ID
Human Swiss Prot
P01861
Formulation
PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Storage and Stability
Store at -20℃/ 1 year. Avoid freeze / thaw cycles.
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Primary Antibodies

    • ELISA Kits

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