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Primary Antibodies
Vitamin D (25-OH-VD)
Vitamin D (25-OH-VD)

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M2335
Reactivity:Species independent
Applications:DB,ELISA
Modifcation:Unmodified
Source:Rabbit
Storage and Stability:Store at -20℃/ 1 year. Avoid freeze / thaw cycles.

$150
# SDS #
Recombinant Antibody
20ul Size Available
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Vitamin D (25-OH-VD)

Western blot analysis of various lysates using TRPM4 Rabbit mAb (M2336) at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (SA0002) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Negative control (NC): HL-60.|Exposure time: 45s.

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Product Details
Product Name
Vitamin D (25-OH-VD)
Description
25-OH-VD, is the primary circulating form of vitamin D in human body after its initial metabolism. It serves as the preferred biomarker for assessing vitamin D status due to its plasma concentration accurately reflecting total body stores. Derived from the hydroxylation of vitamin D precursors (vitamin D3 or D2) in the liver, 25-OH-VD is a precursor to the biologically active 1,25-(OH)2-D3 formed in the kidneys. Its long half-life and stable levels make it the standard analyte for laboratory tests to diagnose vitamin D deficiency or excess, crucial in preventing and treating conditions like osteoporosis, rickets, and various chronic diseases linked to vitamin D, including cardiovascular issues, immune disorders, muscle dysfunction, diabetes, and certain cancers.
Catalog No
M2335
Reactivity
Species independent
Applications
DB, ELISA
Application Dilutions
DB,1:500 - 1:1000 | ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Modifcation
Unmodified
Source
Rabbit
Isotype
IgG
Immunogen
Chemical compounds corresponding to Vitamin D (25-OH-VD)
Calculated MW (kDa)
0
Observed MW (kDa)Gene Name
0VD
Other Name
Gene ID
Human Swiss Prot
Formulation
PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Storage and Stability
Store at -20℃/ 1 year. Avoid freeze / thaw cycles.
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