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Primary Antibodies
Phospho-Myosin Light Chain 2-T18/S19
Phospho-Myosin Light Chain 2-T18/S19

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M2308
Reactivity:Human
Applications:WB,ELISA
Modifcation:Phospho T18/S19
Source:Rabbit
Storage and Stability:Store at -20℃/ 1 year. Avoid freeze / thaw cycles.

$150
# SDS #
Recombinant Antibody
20ul Size Available
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Phospho-Myosin Light Chain 2-T18/S19

Western blot analysis of lysates from HeLa cells, using Phospho-Myosin Light Chain 2-T18 Rabbit mAb (M2309) at 1:1000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (SA0002) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 90s.

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Product Details
Product Name
Phospho-Myosin Light Chain 2-T18/S19
Description
Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene.
Catalog No
M2308
Reactivity
Human
Applications
WB, ELISA
Application Dilutions
WB,1:2000 - 1:7000 | ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Modifcation
Phospho T18/S19
Source
Rabbit
Isotype
IgG
Immunogen
A synthetic phosphorylated peptide around T18/S19 of human Myosin Light Chain 2（NP_006088.2）.
Calculated MW (kDa)
20kDa
Observed MW (kDa)Gene Name
18kDaMYL9
Other Name
LC20; MLC2; MRLC1; MYRL2; MLC-2C; MMIHS4; Phospho-Myosin Light Chain 2-T18/S19
Gene ID
10398
Human Swiss Prot
P24844
Formulation
PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Storage and Stability
Store at -20℃/ 1 year. Avoid freeze / thaw cycles.
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Primary Antibodies

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    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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