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Primary Antibodies
Phospho-eIF4EBP1-T37/T46
Phospho-eIF4EBP1-T37/T46

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M2225
Reactivity:Rat
Applications:WB,ELISA
Modifcation:Phospho T37/T46
Source:Rabbit
Storage and Stability:Store at -20℃/ 1 year. Avoid freeze / thaw cycles.

$150
# SDS #
Recombinant Antibody
20ul Size Available
#
#

Phospho-eIF4EBP1-T37/T46

Western blot analysis of lysates from C6 cells, using Phospho-eIF4EBP1-T37 Rabbit mAb (M2226) at 1:1000 dilution.C6 cells were treated by IGF-1 (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (SA0002) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 60s.

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Product Details
Product Name
Phospho-eIF4EBP1-T37/T46
Description
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Catalog No
M2225
Reactivity
Rat
Applications
WB, ELISA
Application Dilutions
WB,1:2000 - 1:8000 | ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Modifcation
Phospho T37/T46
Source
Rabbit
Isotype
IgG
Immunogen
A synthetic phosphorylated peptide around T37&T46 of human EIF4EBP1.
Calculated MW (kDa)
13kDa
Observed MW (kDa)Gene Name
18-20kDaEIF4EBP1
Other Name
BP-1; 4EBP1; 4E-BP1; PHAS-I; Phospho-eIF4EBP1-T37/T46
Gene ID
1978
Human Swiss Prot
Q13541
Formulation
PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Storage and Stability
Store at -20℃/ 1 year. Avoid freeze / thaw cycles.
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Primary Antibodies

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    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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