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Primary Antibodies
Pseudouridine / 5-ribosyluracil
Pseudouridine / 5-ribosyluracil

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M2193
Reactivity:Species independent
Applications:DB,ELISA
Modifcation:
Source:Rabbit
Storage and Stability:Store at -20℃/ 1 year. Avoid freeze / thaw cycles.

$150
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Recombinant Antibody
20ul Size Available
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Pseudouridine / 5-ribosyluracil

The FITC rabbit monoclonal antibody (M2194) are tested in Western blot against FITC Goat IgG (SA0003),TRITC Goat IgG (SA0490) and Goat IgG (SA0007) at 1:4000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (SA0002) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.

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Product Details
Product Name
Pseudouridine / 5-ribosyluracil
Description
Pseudouridine (Ψ) was among the first post-transcriptional modifications discovered and is overall one of the most abundant（1）. It is present in a wide range of cellular RNAs and is highly conserved across species. Ψ is derived from uridine (U) via base-specific isomerization catalyzed by Ψ synthases. The site-specific pseudouridylation goes through either snoRNA-dependent (requires H/ACA RNP) or -independent mechanism (requires pseudouridine synthase (PUS) family enzymes)（2）. It has an extra hydrogen-bond donor at its non-Watson-Crick edge. When incorporated into RNA, Ψ can alter RNA secondary structure by increasing base stacking, improving base pairing and rigidifying sugar-phosphate backbone5. The chemical and physical properties of RNA can be altered with the incorporation of Ψ, which could contribute to subsequent cellular functions.
Catalog No
M2193
Reactivity
Species independent
Applications
DB, ELISA
Application Dilutions
DB,1:1000 - 1:10000 | ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Modifcation
Source
Rabbit
Isotype
IgG
Immunogen
Chemical compounds corresponding to Pseudouridine / 5-ribosyluracil / Y.
Calculated MW (kDa)
0
Observed MW (kDa)Gene Name
Refer to figuresPseudouridine
Other Name
Gene ID
CAS: 1445-07-4
Human Swiss Prot
Formulation
PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Storage and Stability
Store at -20℃/ 1 year. Avoid freeze / thaw cycles.
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Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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