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Primary Antibodies
UCP1
UCP1

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M2153
Reactivity:Mouse
Applications:WB,ELISA,IHC
Modifcation:Unmodified
Source:Rabbit
Storage and Stability:Store at -20℃/ 1 year. Avoid freeze / thaw cycles.

$150
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Recombinant Antibody
20ul Size Available
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UCP1

Confocal imaging of paraffin-embedded Rat brown adipos tissue using UCP1 Rabbit mAb (M2153, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (SA1138, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

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Product Details
Product Name
UCP1
Description
Mitochondrial uncoupling proteins (UCP) are members of the family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat.
Catalog No
M2153
Reactivity
Mouse
Applications
WB, ELISA,IHC
Application Dilutions
WB,1:5000 - 1:20000 | IHC,1:200 - 1:800 | ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Modifcation
Unmodified
Source
Rabbit
Isotype
IgG
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human UCP1 (NP_068605.1).
Calculated MW (kDa)
33kDa
Observed MW (kDa)Gene Name
33kDaUCP1
Other Name
UCP; SLC25A7; UCP1
Gene ID
7350
Human Swiss Prot
P25874
Formulation
PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Storage and Stability
Store at -20℃/ 1 year. Avoid freeze / thaw cycles.
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Primary Antibodies

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