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Phospho-PAK4/PAK5/PAK6-S474/S560/S602 (AX3246) Rabbit mAb
Phospho-PAK4/PAK5/PAK6-S474/S560/S602 (AX3246) Rabbit mAb

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Catalog No: M1246
Reactivity:Mouse,Rat
Applications:WB,ELISA
Modifcation:Phospho S474/S560/S602
Source:Rabbit
Storage and Stability:Store at -20℃/ 1 year. Avoid freeze / thaw cycles.

$150
# SDS #
Recombinant Antibody
20ul Size Available
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Phospho-PAK4/PAK5/PAK6-S474/S560/S602 (AX3246) Rabbit mAb

Western blot analysis of various lysates using Phospho-PAK4/PAK5/PAK6-S474/S560/S602 Rabbit mAb (M1246) at 1:1000 dilution. Both NIH/3T3 cells and C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (SA0002) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 10s.

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Product Details
Product Name
Phospho-PAK4/PAK5/PAK6-S474/S560/S602 (AX3246) Rabbit mAb
Description
PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Catalog No
M1246
Reactivity
Mouse, Rat
Applications
WB, ELISA
Application Dilutions
WB,1:500 - 1:1000 | ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Modifcation
Phospho S474/S560/S602
Source
Rabbit
Isotype
IgG
Immunogen
A synthetic phosphorylated peptide around S474 of human PAK4PAK4 (O96013).
Calculated MW (kDa)
64, 80, 75 kDa
Observed MW (kDa)Gene Name
64kDa,75kDa,70kDaPAK4/PAK5/PAK6
Other Name
PAK4; KIAA1142; PAK5KIAA1264; PAK6; Phospho-PAK4/PAK5/PAK6-S474/S560/S602
Gene ID
10298/57144/56924
Human Swiss Prot
O96013(PAK4-Antigen),Q9P286(PAK5),Q9NQU5(PAK6)
Formulation
PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Storage and Stability
Store at -20℃/ 1 year. Avoid freeze / thaw cycles.
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Primary Antibodies

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