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Primary Antibodies
Phospho-SIRT1-S47 Rabbit mAb
Phospho-SIRT1-S47 Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M1197
Reactivity:Human
Applications:WB,ELISA
Modifcation:Phospho S47
Source:Rabbit
Storage and Stability:Store at -20℃/ 1 year. Avoid freeze / thaw cycles.

$150
# SDS #
Recombinant Antibody
20ul Size Available
#
#

Phospho-SIRT1-S47 Rabbit mAb

Western blot analysis of lysates from 293T cells, using Phospho-SIRT1-S47 Rabbit mAb (AP0976) at 1:500 dilution. 293T cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.

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Product Details
Product Name
Phospho-SIRT1-S47 Rabbit mAb
Description
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants.
Catalog No
M1197
Reactivity
Human
Applications
WB, ELISA
Application Dilutions
WB,1:100 - 1:500 | ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Modifcation
Phospho S47
Source
Rabbit
Isotype
IgG
Immunogen
A synthetic phosphorylated peptide around S47 of human SIRT1 (Q96EB6).
Calculated MW (kDa)
82kDa
Observed MW (kDa)Gene Name
120kDaSIRT1
Other Name
SIR2; SIR2L1; SIR2alpha; Phospho-SIRT1-S47
Gene ID
23411
Human Swiss Prot
Q96EB6
Formulation
PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Storage and Stability
Store at -20℃/ 1 year. Avoid freeze / thaw cycles.
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Primary Antibodies

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    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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