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Primary Antibodies
DDDDK-Tag(binds to flag sequnence) Antibody (12M11)
DDDDK-Tag(binds to flag sequnence) Antibody (12M11)

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: N2010
Reactivity:Species independent
Applications:WB,ELISA,IP,IF,CoIP
Modification:Unmodified/Total
Source:Store at -20°C/1 year
Storage and Stability:

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DDDDK-Tag(binds to flag sequnence) Antibody (12M11)

Immunofluorescence analysis of 293T transfected FLAG and GFP tag protein. 1, FLAG primary Antibody(red N2010) was diluted at 1:1000(4°C overnight), GFP primary Antibody(green C40014) was diluted at 1:1000(4°C overnight),

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Product Details
Product Name
DDDDK-Tag(binds to flag sequnence) Antibody (12M11)
Description
Catalog No
N2010
Reactivity
Species independent
Source
Mouse VH IgG1 and VL Kappa was expressed from 293F cells
Modification
Unmodified/Total
Applications
WB, ELISA, IP, IF, CoIP
Dilution
WB 1:10000-50000 ELISA 1:20000-50000 IP 1:50-1:200 IF 1:1000 -3000
Purification
Protein A purified
Concentration
Storage and Stability
Store at -20°C/1 year
Other Name
Molecular Weight (Da or kDa)
Gene Name
Flag tag; Flag-tag,DDDDK TAG, DDDDK-TAG, DYKDDDDK tag,DYKDDDDK-tag
Protein Name
Human Gene ID
Synthetic Peptide of Flag-Tag
Human Swiss Prot No.
This recombinant antibody can highly specifically recognize the Flag tags of C-terminal and N-terminal of recombinant protein. Variable region gene of this antibody comes from the mouse gene library immunized with DDDK peptide. Constant region of this antibody is mouse IgG1 and mouse kappa.
Immunogen
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Specificity
Formulation
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Publications (14)
Pathogenic variants in TSC2 might cause premature ovarian insufficiency through activated mTOR induced hyperactivation of primordial follicles

Author: Available in link provided

Date of Publication : Available in link provided

Tat-hspb1 Suppresses Clear Cell Renal Cell Carcinoma (ccRCC) Growth via Lysosomal Membrane Permeabilization

Author: Available in link provided

Date of Publication : Available in link provided

circEXOC6B interacting with RRAGB, an mTORC1 activator, inhibits the progression of colorectal cancer by antagonizing the HIF1A-RRAGB-mTORC1 positive feedback loop IF,CoIP,RIP Human /SW620, HCT116 Cells

Author: Available in link provided

Date of Publication : Available in link provided

SLC35E2 promoter mutation as a prognostic marker of esophageal squamous cell carcinoma Life Sci. 2022 May;296:120447. IF Human 1:2000

Author: Available in link provided

Date of Publication : Available in link provided

The deubiquitinase USP6 affects memory and synaptic plasticity through modulating NMDA receptor stability. PLOS BIOLOGY Plos Biol. 2019 Dec;17(12):e3000525 CoIP Human,Mouse 1:3000 Brain HEK293T cell

Author: Available in link provided

Date of Publication : Available in link provided

miR-375 is involved in Hippo pathway by targeting YAP1/TEAD4-CTGF axis in gastric carcinogenesis. Cell Death & Disease 2018 Jan 24 WB Human AGS cell

Author: Available in link provided

Date of Publication : Available in link provided

Salmonella Effector SpvB Inhibits NF-κB Activity via KEAP1-Mediated Downregulation of IKKβ. Frontiers in Cellular and Infection Microbiology Front Cell Infect Mi. 2021 Mar;11:207 WB Human HeLa cells，293T cells

Author: Available in link provided

Date of Publication : Available in link provided

Identification of the internal ribosome entry sites in the 5'‑untranslated region of the c‑fos gene. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Int J Mol Med. 2021 Apr;47(4):1-1 IP Human 1: 5000 293T cell

Author: Available in link provided

Date of Publication : Available in link provided

Engineered zinc-finger transcription factors inhibit the replication and transcription of HBV in vitro and in vivo. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Int J Mol Med. 2018 Apr;41(4):2169-2176 IF Human 1:1000 HepG2.2.15 cell

Author: Available in link provided

Date of Publication : Available in link provided

CMTM7 inhibits breast cancer progression by regulating Wnt/β-catenin signaling BREAST CANCER RESEARCH Yue Yu CoIP Human MCF-7 cell

Author: Available in link provided

Date of Publication : Available in link provided

Microtubule-associated protein 4 promotes epithelial mesenchymal transition in hepatocellular cancer cells via regulating GSK3β/β-catenin pathway Heliyon Pingping Hu WB Human MHCC97-L cell

Author: Available in link provided

Date of Publication : Available in link provided

Mutant p53 activates hnRNPA2B1-AGAP1-mediated exosome formation to promote esophageal squamous cell carcinoma progression CANCER LETTERS Zhihua Liu WB Human KYSE510 cell

Author: Available in link provided

Date of Publication : Available in link provided

RPS15 interacted with IGF2BP1 to promote esophageal squamous cell carcinoma development via recognizing m6A modification. Zhihua Liu WB,IP Human 1:5000 HEK 293 T cell,KYSE30 cell,KYSE450 cell

Author: Available in link provided

Date of Publication : Available in link provided

Cystathionine β-synthase (CBS) suppresses NLRP3 inflammasome activation via redox regulation in microglia. Dr. Lifang Hu WB Mouse 1:1000 BV2 cell

Author: Available in link provided

Date of Publication : Available in link provided

Primary Antibodies

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    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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