actin beta(ACTB)Homo sapiens. This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008],
Catalog No
N0012
Reactivity
Human,Mouse,Rat,Hamster,Monkey
Applications
WB,IF/ICC,FCM,ELISA
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. Flow cytometry: 1/200 - 1/400. ELISA: 1/10000. Not yet tested in other applications.
Purification
Affinity purification
Concentration
Storage and Stability
-20°C/1 year
Other Name
ACTB; Actin; cytoplasmic 1; Beta-actin
Molecular Weight (Da)
Gene Name
ACTB
Protein Name
Actin cytoplasmic 1
Human Gene ID
60
Human Swiss Prot No.
P60709
Immunogen
Synthetic peptide corresponding to amino-terminal residues of human Actin β, conjugated to KLH.
Confocal immunofluorescence analysis of SKBR-3 (left) and A549 (right) cells using Actin β Monoclonal Antibody (red. the secondary Ab is Cy3-Goat anti mouse IgG). Blue: DRAQ5 fluorescent DNA dye.
Western Blot analysis using Actin β Monoclonal Antibody against NIH/3T3 (1). Jurkat (2). HeLa (3). CHO (4). PC12 (5). HEK293 (6). COS (7). A549 (8) and MCF-7 (9) cell lysate.
