Description
alpha fetoprotein(AFP) Homo sapiens. This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008],
Reactivity
Human
Applications
WB,ELISA
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
Affinity purification
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
AFP; HPAFP; Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin
Molecular Weight (Da)
Gene Name
AFP
Protein Name
Alpha-fetoprotein
Human Gene ID
174
Human Swiss Prot No.
P02771
Immunogen
Purified recombinant fragment of human AFP expressed in E. Coli.
Specificity
AFP Monoclonal Antibody detects endogenous levels of AFP protein.
Formulation
Antibody are purified by protein G affinity chromatography. Liquid in PBS containing 50% glycerol and 0.03% sodium azide.
