alpha-methylacyl-CoA racemase(AMACR) Homo sapiens. This gene encodes a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described. Read-through transcription also exists between this gene and the upstream neighboring C1QTNF3 (C1q and tumor necrosis factor related protein 3) gene. [provided by RefSeq, Mar 2011],
Catalog No
N0023
Reactivity
Human,Mouse
Applications
WB,IHC-p,IF/ICC,ELISA
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/200 - 1/1000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Confocal immunofluorescence analysis of LNCaP cells using AMACR Monoclonal Antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry analysis of paraffin-embedded human normal prostate tissues (left) and prostate adenocarcinoma tissues (right). showing cytoplasmic localization with DAB staining using AMACR Monoclonal Antibody.
