keratin 19(KRT19)Homo sapiens. The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008],
Catalog No
N0182
Reactivity
Human
Applications
WB,IHC-p,IF(paraffin section),ELISA
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification
Affinity purification
Concentration
Storage and Stability
-20°C/1 year
Other Name
KRT19; Keratin; type I cytoskeletal 19; Cytokeratin-19; CK-19; Keratin-19; K19
Molecular Weight (Da)
Gene Name
KRT19
Protein Name
Keratin type I cytoskeletal 19
Human Gene ID
3880
Human Swiss Prot No.
P08727
Immunogen
Purified recombinant fragment of Cytokeratin 19 (aa80-400) expressed in E. Coli.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma(A). lung cancer(B) and normal colon tissue(C). showing cytoplasmic localization with DAB staining using Cytokeratin 19 Monoclonal Antibody.
Western Blot analysis using Cytokeratin 19 Monoclonal Antibody against truncated KRT19-His recombinant protein (1) and full-length KRT19(aa1-400)-hIgGFc transfected CHO-K1 cell lysate(2).