keratin 8(KRT8)Homo sapiens. This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012],
Catalog No
N0189
Reactivity
Human
Applications
WB,IHC-p,IF/ICC,ELISA
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/200 - 1/1000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification
Affinity purification
Concentration
Storage and Stability
-20°C/1 year
Other Name
KRT8; CYK8; Keratin; type II cytoskeletal 8; Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8
Molecular Weight (Da)
Gene Name
KRT8
Protein Name
Keratin type II cytoskeletal 8
Human Gene ID
3856
Human Swiss Prot No.
P05787
Immunogen
Purified recombinant fragment of human Cytokeratin 8 (aa391-483) expressed in E. Coli.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma (A). lung cancer (B) and ovarian cancer tissue (C). showing membrane and cytoplasmic localization with DAB staining using Cytokeratin 8 Monoclonal Antibody.
