paternally expressed 10(PEG10) Homo sapiens. This is a paternally expressed imprinted gene that is thought to have been derived from the Ty3/Gypsy family of retrotransposons. It contains two overlapping open reading frames, RF1 and RF2, and expresses two proteins: a shorter, gag-like protein (with a CCHC-type zinc finger domain) from RF1; and a longer, gag/pol-like fusion protein (with an additional aspartic protease motif) from RF1/RF2 by -1 translational frameshifting (-1 FS). While -1 FS has been observed in RNA viruses and transposons in both prokaryotes and eukaryotes, this gene represents the first example of -1 FS in a eukaryotic cellular gene. This gene is highly conserved across mammalian species and retains the heptanucleotide (GGGAAAC) and pseudoknot elements required for -1 FS. It is expressed in adult and embryonic tissues (most notably in placenta) and reported to have a role in cell proliferation, differentiation and apoptosis.
Catalog No
N0514
Reactivity
Human
Applications
WB,IHC-p,IF/ICC,ELISA
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/200 - 1/1000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification
Affinity purification
Concentration
Storage and Stability
-20°C/1 year
Other Name
PEG10; EDR; KIAA1051; MAR2; MART2; MEF3L1; RGAG3; Retrotransposon-derived protein PEG10; Embryonal carcinoma differentiation-regulated protein; Mammalian retrotransposon-derived protein 2; Myelin expression factor 3-like protein 1; MEF3-lik
Molecular Weight (Da)
Gene Name
PEG10
Protein Name
Retrotransposon-derived protein PEG10
Human Gene ID
23089
Human Swiss Prot No.
Q86TG7
Immunogen
Purified recombinant fragment of PEG10 (aa1-120) expressed in E. Coli.
Specificity
PEG10 Monoclonal Antibody detects endogenous levels of PEG10 protein.
Western Blot analysis using PEG10 Monoclonal Antibody against truncated Trx-PEG10 recombinant protein (1).truncated GST-PEG10 (aa1-120) recombinant protein (2) and full-length PEG10 (aa1-325)-hIgGFc transfected CHO-K1 cell lysate (3).
