SMAD family member 5(SMAD5) Homo sapiens. The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014],
Catalog No
N0583
Reactivity
Human,Rat
Applications
WB,IHC-p,IF/ICC,FCM,ELISA
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/200 - 1/1000. Immunofluorescence: 1/200 - 1/1000. Flow cytometry: 1/200 - 1/400. ELISA: 1/10000. Not yet tested in other applications.
Purification
Affinity purification
Concentration
Storage and Stability
-20°C/1 year
Other Name
SMAD5; MADH5; Mothers against decapentaplegic homolog 5; MAD homolog 5; Mothers against DPP homolog 5; JV5-1; SMAD family member 5; SMAD 5; Smad5; hSmad5
Molecular Weight (Da)
Gene Name
SMAD5
Protein Name
Mothers against decapentaplegic homolog 5
Human Gene ID
4090
Human Swiss Prot No.
Q99717
Immunogen
Purified recombinant fragment of human Smad5 expressed in E. Coli.
Specificity
Smad5 Monoclonal Antibody detects endogenous levels of Smad5 protein.
Immunofluorescence analysis of NTERA-2 cells using Smad5 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry analysis of paraffin-embedded brain tissues (left) and lung cancer tissues (right) with DAB staining using Smad5 Monoclonal Antibody.