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Primary Antibodies
Flag-Tag Monoclonal Antibody(2C5)
Flag-Tag Monoclonal Antibody(2C5)

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: N1265
Reactivity:Species independent
Applications:WB,IP,IF/ICC
Modification:Unmodfied/ Total
Source:Monoclonal Mouse
Storage and Stability:-20°C/1 year

$200
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Flag-Tag Monoclonal Antibody(2C5)

1ug Flag fusion protein+ Primary antibody dilution at 1:10000

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Product Details
Product Name
Flag-Tag Monoclonal Antibody(2C5)
Description
The DYKDDDDK peptide (Flag-tag) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, over expressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
Catalog No
N1265
Reactivity
Species independent
Applications
WB,IP,IF/ICC
Modification
Unmodfied/ Total
Source
Monoclonal Mouse
Dilution
WB: 1:5000 IP: 1:200 IF: 1:2000
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration
Storage and Stability
-20°C/1 year
Other Name
Molecular Weight (Da)
Gene Name
Flag Tag
Protein Name
Flag-Tag DDDDK TAG, DDDDK-TAG
Human Gene ID
Human Swiss Prot No.
Immunogen
Synthetic Peptide of Flag-Tag
Specificity
The antibody detects C-terminal, internal, and N-terminal Flag-tag fusion proteins.
Formulation
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
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Publications (3)
circEXOC6B interacting with RRAGB, an mTORC1 activator, inhibits the progression of colorectal cancer by antagonizing the HIF1A-RRAGB-mTORC1 positive feedback loop

Author: Li, Xiaomin, Wang, Jianjun, Lin, Weihao, Yuan, Qinzi, Lu, Yanxia, Wang, Haowei, Chen, Yujia, Chen, Lixia, Dai, Peiling, Long, Huaicheng, Li, Xuenong

Date of Publication : 2022-06-23

Engineered zinc-finger transcription factors inhibit the replication and transcription of HBV in vitro and in vivo

Author: Wei Luo, Junxia Wang, Dengfeng Xu, Huili Bai, Yangli Zhang, Yuhong Zhang, Xiaosong Li

Date of Publication : 2018-04-01

SLC35E2 promoter mutation as a prognostic marker of esophageal squamous cell carcinoma

Author: Yang Li, Riyue Feng, Xiao Yu, Lei Li, Yuhao Liu, Ruixiang Zhang, Xiankai Chen, Yahui Zhao, Zhihua Liu

Date of Publication : 44622

Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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