Wilms' Tumor 1(WT1) (PT0264) mouse mAb

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Catalog No: N1769

Reactivity:Human,Mouse

Applications:IHC-p,IF(paraffin section)

Modification:Unmodfied/ Total

Source:Monoclonal Mouse/IgG1,Kappa

Storage and Stability:-20°C/1 year

Size:

Price/Quantity $190

Quantity:

Datasheet

SDS

Immunohistochemical

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Wilms' Tumor 1(WT1) (PT0264) mouse mAb

Immunohistochemical analysis of paraffin-embedded Mesothelioma. 1. Antibody was diluted at 1:200(4° overnight). 2. TRIS-EDTA of pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).

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Lead Time

Antibodies 1-2 business day, ELISA kits 2-3 business day lead time

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Product Details

Product Name

Wilms' Tumor 1(WT1) (PT0264) mouse mAb

Description

Wilms tumor 1(WT1) Homo sapiens. This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Mar 2015],

Catalog No

N1769

Reactivity

Human, Mouse

Applications

IHC-p,IF(paraffin section)

Modification

Unmodfied/ Total

Source

Monoclonal Mouse/IgG1, Kappa

Dilution

IHC-p 1:100-500，WB 1:500-2000

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

Storage and Stability

-20°C/1 year

Other Name

Molecular Weight (Da)

Gene Name

WT1

Protein Name

Wilms tumor protein (WT33)

Human Gene ID

7490

Human Swiss Prot No.

P19544

Immunogen

Synthesized peptide derived from human Wilms' Tumor 1(WT1)

Specificity

This antibody detects endogenous levels of human Wilms' Tumor 1(WT1). Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH8.0 was highly recommended as antigen repair method in paraffin section

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.