CD14 (PT0020) mouse mAb

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Catalog No: N1772

Reactivity:Human,Mouse

Applications:IHC-p,IF(paraffin section),mult-IHC

Modification:Unmodfied/ Total

Source:Monoclonal Mouse/IgG2b,Kappa

Storage and Stability:-20°C/1 year

Size:

Price/Quantity $190

Quantity:

Datasheet

SDS

Immunohistochemical

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CD14 (PT0020) mouse mAb

Fluorescence multiplex immunohistochemical analysis of normal human gastric cancer tissue (formalin-fixed paraffin-embedded section).The section was incubated in 3 rounds of staining; in the order of CD15 R12-2147 (1/50 dilution). CD11b C30326 (1/50 dilution). CD14 N1772 (1/50 dilution).each using a separate fluorescent tyramide signal amplification system.Sodium citrate antigen retrieval (Assay Biotech YS0002. 30 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round. to avoid any cross-reactivity.DAPI (dark blue) was used as a nuclear counter stain.

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Lead Time

Antibodies 1-2 business day, ELISA kits 2-3 business day lead time

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Product Details

Product Name

CD14 (PT0020) mouse mAb

Description

CD14 molecule(CD14) Homo sapiens. The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Mar 2010],

Catalog No

N1772

Reactivity

Human, Mouse

Applications

IHC-p,IF(paraffin section),mult-IHC

Modification

Unmodfied/ Total

Source

Monoclonal Mouse/IgG2b, Kappa

Dilution

IHC-p 1:100-500

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

Storage and Stability

-20°C/1 year

Other Name

Molecular Weight (Da)

Gene Name

CD14

Protein Name

Monocyte differentiation antigen CD14 (Myeloid cell-specific leucine-rich glycoprotein) (CD antigen CD14) [Cleaved into: Monocyte differentiation antigen CD14, urinary form; Monocyte differentiation antigen CD14, membrane-bound form]

Human Gene ID

929

Human Swiss Prot No.

P08571

Immunogen

Synthesized peptide derived from human CD14

Specificity

This antibody detects endogenous levels of human CD14. Heat-induced epitope retrieval (HIER) Citrate buffer of pH6.0 was highly recommended as antigen repair method in paraffin section

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.