cyclin dependent kinase inhibitor 1C(CDKN1C) Homo sapiens. This gene is imprinted, with preferential expression of the maternal allele. The encoded protein is a tight-binding, strong inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndorome, suggesting that this gene is a tumor suppressor candidate. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2010],
Catalog No
N1790
Reactivity
Human, Mouse
Applications
IHC-p,IF(paraffin section)
Modification
Unmodfied/ Total
Source
Monoclonal Mouse/IgG3, Kappa
Dilution
WB 500-2000 IHC-p 1:100-500
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
This antibody detects endogenous levels of human p57kip2. Heat-induced epitope retrieval (HIER) Citrate buffer of pH6.0 was highly recommended as antigen repair method in paraffin section
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Immunohistochemical analysis of paraffin-embedded Placenta. 1. Antibody was diluted at 1:200(4° overnight). 2. Citrate buffer of pH6.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).
Immunohistochemical analysis of paraffin-embedded Placenta. 1. Antibody was diluted at 1:200(4° overnight). 2. Citrate buffer of pH6.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).
Immunohistochemical analysis of paraffin-embedded Partial hydatidiform mole. 1. Antibody was diluted at 1:200(4° overnight). 2. Citrate buffer of pH6.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).
