Proliferating Cell Nuclear Antigen (PCNA) (PT0221) mouse mAb

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Catalog No: N1792

Reactivity:Human,Mouse

Applications:IHC-p,IF(paraffin section)

Modification:Unmodfied/ Total

Source:Monoclonal Mouse/IgG1,Kappa

Storage and Stability:-20°C/1 year

Size:

Price/Quantity $190

Quantity:

Datasheet

SDS

Immunohistochemical

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Proliferating Cell Nuclear Antigen (PCNA) (PT0221) mouse mAb

Immunohistochemical analysis of paraffin-embedded Squamous cell lung carcinoma. 1. Antibody was diluted at 1:200(4° overnight). 2. TRIS-EDTA of pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).

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Antibodies 1-2 business day, ELISA kits 2-3 business day lead time

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Product Details

Product Name

Proliferating Cell Nuclear Antigen (PCNA) (PT0221) mouse mAb

Description

proliferating cell nuclear antigen(PCNA)Homo sapiens. The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008],

Catalog No

N1792

Reactivity

Human, Mouse

Applications

IHC-p,IF(paraffin section)

Modification

Unmodfied/ Total

Source

Monoclonal Mouse/IgG1, Kappa

Dilution

IHC-p 1:100-500，WB 1:500-2000

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

Storage and Stability

-20°C/1 year

Other Name

Molecular Weight (Da)

Gene Name

PCNA

Protein Name

Proliferating cell nuclear antigen (PCNA) (Cyclin)

Human Gene ID

5111

Human Swiss Prot No.

P12004

Immunogen

Synthesized peptide derived from human Proliferating Cell Nuclear Antigen (PCNA)

Specificity

This antibody detects endogenous levels of human Proliferating Cell Nuclear Antigen (PCNA). Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH8.0 was highly recommended as antigen repair method in paraffin section

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.