topoisomerase (DNA) II alpha(TOP2A) Homo sapiens. This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also pla
Catalog No
N1808
Reactivity
Human, Mouse
Applications
IHC-p,IF/ICC
Modification
Unmodfied/ Total
Source
Monoclonal Mouse/IgG2b, Kappa
Dilution
IHC-p 1:100-500，WB 1:500-2000，IF 1:500-200
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration
Storage and Stability
-20°C/1 year
Other Name
Molecular Weight (Da)
Gene Name
TOP2A TOP2
Protein Name
DNA topoisomerase 2-alpha (EC 5.99.1.3) (DNA topoisomerase II, alpha isozyme)
Human Gene ID
7153
Human Swiss Prot No.
P11388
Immunogen
Synthesized peptide derived from human Topoisomerase IIα
Specificity
This antibody detects endogenous levels of human Topoisomerase IIα. Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH8.0 was highly recommended as antigen repair method in paraffin section
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Immunohistochemical analysis of paraffin-embedded human Transitional cell carcinoma of the urinary bladder Antibody was diluted at 1:200(4° overnight).
Immunohistochemical analysis of paraffin-embedded Tonsil. 1. Antibody was diluted at 1:200(4° overnight). 2. TRIS-EDTA of pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).
Immunohistochemical analysis of paraffin-embedded Colon. 1. Antibody was diluted at 1:200(4° overnight). 2. TRIS-EDTA of pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).
Immunohistochemical analysis of paraffin-embedded Colon. 1. Antibody was diluted at 1:200(4° overnight). 2. TRIS-EDTA of pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).
Immunohistochemical analysis of paraffin-embedded Colon. 1. Antibody was diluted at 1:200(4° overnight). 2. TRIS-EDTA of pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).
