Topoisomerase IIα (PT0247) mouse mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: N1808

Reactivity:Human,Mouse

Applications:IHC-p,IF/ICC

Modification:Unmodfied/ Total

Source:Monoclonal Mouse/IgG2b,Kappa

Storage and Stability:-20°C/1 year

Size:

Price/Quantity $190

Quantity:

Datasheet

SDS

Immunohistochemical

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Topoisomerase IIα (PT0247) mouse mAb

Immunohistochemical analysis of paraffin-embedded Colon. 1. Antibody was diluted at 1:200(4° overnight). 2. TRIS-EDTA of pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).

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Lead Time

Antibodies 1-2 business day, ELISA kits 2-3 business day lead time

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Product Details

Product Name

Topoisomerase IIα (PT0247) mouse mAb

Description

topoisomerase (DNA) II alpha(TOP2A) Homo sapiens. This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also pla

Catalog No

N1808

Reactivity

Human, Mouse

Applications

IHC-p,IF/ICC

Modification

Unmodfied/ Total

Source

Monoclonal Mouse/IgG2b, Kappa

Dilution

IHC-p 1:100-500，WB 1:500-2000，IF 1:500-200

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

Storage and Stability

-20°C/1 year

Other Name

Molecular Weight (Da)

Gene Name

TOP2A TOP2

Protein Name

DNA topoisomerase 2-alpha (EC 5.99.1.3) (DNA topoisomerase II, alpha isozyme)

Human Gene ID

7153

Human Swiss Prot No.

P11388

Immunogen

Synthesized peptide derived from human Topoisomerase IIα

Specificity

This antibody detects endogenous levels of human Topoisomerase IIα. Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH8.0 was highly recommended as antigen repair method in paraffin section

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.