actin beta(ACTB)Homo sapiens. This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008],
Catalog No
N1814
Reactivity
Human, Mouse
Applications
IHC-p,IF(paraffin section)
Modification
Unmodfied/ Total
Source
Monoclonal Mouse/IgG2b, Kappa
Dilution
IHC-p 1:100-500，WB 1:500-2000
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration
Storage and Stability
-20°C/1 year
Other Name
Molecular Weight (Da)
Gene Name
Actin pan
Protein Name
Actin pan
Human Gene ID
Human Swiss Prot No.
P60709/Q9BYX7/P63261
Immunogen
Synthesized peptide derived from human Actin pan
Specificity
This antibody detects endogenous levels of human Actin pan. Heat-induced epitope retrieval (HIER) Citrate buffer of pH6.0 was highly recommended as antigen repair method in paraffin section
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Immunohistochemical analysis of paraffin-embedded Skeletal muscle. 1. Antibody was diluted at 1:200(4° overnight). 2. Citrate buffer of pH6.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).
Immunohistochemical analysis of paraffin-embedded Colon. 1. Antibody was diluted at 1:200(4° overnight). 2. Citrate buffer of pH6.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).
Immunohistochemical analysis of paraffin-embedded Colon. 1. Antibody was diluted at 1:200(4° overnight). 2. Citrate buffer of pH6.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).
Immunohistochemical analysis of paraffin-embedded Colon. 1. Antibody was diluted at 1:200(4° overnight). 2. Citrate buffer of pH6.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).
