claudin 3(CLDN3)Homo sapiens. Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this intronless gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is also a low-affinity receptor for Clostridium perfringens enterotoxin, and shares aa sequence similarity with a putative apoptosis-related protein found in rat. [provided by RefSeq, Jul 2008],
Catalog No
N1829
Reactivity
Human, Mouse
Applications
IHC-p,IF(paraffin section)
Modification
Unmodfied/ Total
Source
Monoclonal Mouse/IgG1, Kappa
Dilution
WB 500-2000 IHC-p 1:100-500
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
This antibody detects endogenous levels of human Claudin 3. Heat-induced epitope retrieval (HIER) Citrate buffer of pH6.0 was highly recommended as antigen repair method in paraffin section
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Immunohistochemical analysis of paraffin-embedded Rectum. 1. Antibody was diluted at 1:200(4° overnight). 2. Citrate buffer of pH6.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).
Immunohistochemical analysis of paraffin-embedded Colon. 1. Antibody was diluted at 1:200(4° overnight). 2. Citrate buffer of pH6.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).
Immunohistochemical analysis of paraffin-embedded Colon. 1. Antibody was diluted at 1:200(4° overnight). 2. Citrate buffer of pH6.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).
