keratin 10(KRT10)Homo sapiens. This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. [provided by RefSeq, Jul 2008],
Catalog No
N1833
Reactivity
Human, Mouse
Applications
IHC-p,IF(paraffin section)
Modification
Unmodfied/ Total
Source
Monoclonal Mouse/IgG1, Kappa
Dilution
WB 500-2000 IHC-p 1:100-500
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration
Storage and Stability
-20°C/1 year
Other Name
Keratin, type I cytoskeletal 10 (Cytokeratin-10) (CK-10) (Keratin-10) (K10)
Molecular Weight (Da)
Gene Name
KRT10 KPP
Protein Name
Cytokeratin 10
Human Gene ID
3858
Human Swiss Prot No.
P13645
Immunogen
Synthesized peptide derived from human Cytokeratin 10
Specificity
This antibody detects endogenous levels of human Cytokeratin 10. Heat-induced epitope retrieval (HIER) Citrate buffer of pH6.0 was highly recommended as antigen repair method in paraffin section
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Immunohistochemical analysis of paraffin-embedded human Cutaneous Squamous Cell carcinoma. 1. Antibody was diluted at 1:200(4° overnight). 2. Tris-EDTA.pH9.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).
