catenin beta 1(CTNNB1) Homo sapiens. The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016],
Catalog No
N1868
Reactivity
Human, Mouse
Applications
IHC-p,IF(paraffin section)
Modification
Unmodfied/ Total
Source
Monoclonal Mouse/IgG2b, Kappa
Dilution
IHC-p 1:100-500，WB 1:500-2000
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration
Storage and Stability
-20°C/1 year
Other Name
Molecular Weight (Da)
Gene Name
CTNNB1 CTNNB OK/SW-cl.35 PRO2286
Protein Name
Catenin beta-1 (Beta-catenin)
Human Gene ID
1499
Human Swiss Prot No.
P35222
Immunogen
Synthesized peptide derived from human Beta-catenin
Specificity
This antibody detects endogenous levels of human Catenin beta-1 (Beta-catenin). Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH9.0 was highly recommended as antigen repair method in paraffin section
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Immunohistochemical analysis of paraffin-embedded human Liver. 1.Beta-catenin Antibody was diluted at 1:200(4° overnight). 2. EDTA pH 9.0 was used for antigen retrieval
Immunohistochemical analysis of paraffin-embedded human Colon carcinoma. 1.Beta-catenin Antibody was diluted at 1:200(4° overnight). 2. EDTA pH 9.0 was used for antigen retrieval
Ma. Zhan. Shuping Lou. and Zheng Jiang. "PHLDA2 regulates EMT and autophagy in colorectal cancer via the PI3K/AKT signaling pathway." Aging (Albany NY) 12.9 (2020): 7985.
