Vimentin is the most common member of intermediate filament protein family. It is a main component of cytoskeleton structure. Its main function is to maintain cell integrity and skeleton stability. It is expressed in mesenchymal cells and mesoderm derived cells. Vimentin is a relatively specific marker of mesenchymal cells and their derived tumors.
Catalog No
V0475
Reactivity
Human
Application
IHC,WB
Modification
Unmodified/ Total
Source
Monoclonal Mouse
Storage and Stability
Stable at -20°C for at least 1 year.
Other Name
CTRCT30, Epididymis luminal protein 113, FLJ36605, HEL113, VIM, VIME_HUMAN, Vimentin
Gene Name
VIM
UniProtKB
P08670
Clone ID
ABT019
Isotype
IgG2b, Kappa
Localization
Cytoplasmic
Positive Tissue Control
Tonsil/ Liver
Specificity
The antibody can specifically recognize human Vimentin protein. In western blotting of HEK293 cell lysate, the antibody can label a 54 kDa band corresponding to Vimentin.
Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Vimentin and anti-GAPDH antibody. The HRP-conjugated anti-Mouse IgG antibody was used to detect the antibody.
Lane 1: Wide type HEK293 cell lysate
Lane 2: Vimentin knockout HEK293 cell lysate
Predicted band size: 54kDa
Observed band size: 54kDa