P53 is a tumor suppressor gene located on chromosome 17. The mutation of p53 gene will change its spatial conformation and turn into oncogene, and then lose the regulation of cell growth, apoptosis and DNA repair. The mutation or deletion of p53 gene is the cause of many tumors. Compared with the wild-type protein, the mutant p53 protein has a longer half-life, so it is easier to detect by immunohistochemistry. The p53 mutant protein is expressed in many kinds of tumors, such as breast cancer, gastrointestinal cancer, hepatocellular carcinoma and respiratory tract tumor. It is mainly used for the study of various tumors and can be used as an indicator of tumor prognosis.
Catalog No
V0429
Reactivity
Human
Application
IHC,WB
Modification
Unmodified/ Total
Source
Monoclonal Mouse
Storage and Stability
Stable at -20°C for at least 1 year.
Other Name
Antigen NY-CO-13, BCC7, Cellular tumor antigen p53, FLJ92943, LFS1, Mutant tumor protein 53, p53, p53 tumor suppressor, P53_HUMAN, Phosphoprotein p53, Tp53, Transformation related protein 53, TRP53, tumor antigen p55, Tumor protein 53, Tumor protein p53, Tumor suppressor p53
The antibody can recognize human wild type and mutant P53 protein. In western blotting of wild type HEK293 cell lysate, the antibody can label a 50 kDa band corresponding to P53, while there is no band detected in P53 knock out HEK293 cell lysate.
P53 knockout and wild-type HEK93 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-P53 and anti-GAPDH antibody. The HRP-conjugated anti-Mouse IgG antibody was used to detect the antibody.
Lane1: Wild type HEK293 whole cell lysate, 20ug;
Lane2: P53 knockout HEK293 whole cell lysate, 20ug;
Predicted band size: 53 kDa
Observed band size: 53 kDa
