The PKD1 (Phospho-Ser910) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect PKD1 (Phospho-Ser910) protein expression profile in cells. The kit can be used for measuring the relative amounts of PKD1 (Phospho-Ser910) in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on PKD1 (Phospho-Ser910).
This kit contains Crystal Violet Staining normalization protocol. It provides a means to normalize Absorbance 450 nm of primary antibodies to cell density of the cells in the same well. This provides an accurate account for stimulations and cell densities during the assay. By comparing the proportional values obtained from the experimental wells to those of the control wells (e.g. untreated cells), it is possible to determine the extent to which the stimulant(s) have affected the expression levels.
Catalog No
CBP2113
Format
Two 96-Well Plates
Detection Target
PKD1 (Phospho-Ser910)
Reactivity
Human, Mouse, Rat
Detection Method
Colorimetric 450 nm
Assay Type
Cell-Based ELISA
SwissProt ID
Q15139
NCBI Gene ID
5587
Target Synonyms
PRKD1; PKD; PKD1; PRKCM; Serine/threonine-protein kinase D1; Protein kinase C mu type; Protein kinase D; nPKC-D1; nPKC-mu
