PRKD1 is a serine/threonine kinase that regulates a variety of cellular functions, including membrane receptor signaling, transport at the Golgi, protection from oxidative stress at the mitochondria, gene transcription, and regulation of cell shape, motility, and adhesion (summary by Eiseler et al., 2009 [PubMed 19329994]).[supplied by OMIM, Nov 2010]
Catalog No
A0806
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
PRKD1; PKD; PKD1; PRKCM; Serine/threonine-protein kinase D1; Protein kinase C mu type; Protein kinase D; nPKC-D1; nPKC-mu
Molecular Weight (Da)
101888
Gene Name
PRKD1
Protein Name
Serine/threonine-protein kinase D1
Human Gene ID
5587
Human Swiss Prot No.
Q15139
Immunogen
The antiserum was produced against synthesized peptide derived from human PKD1/PKC mu around the phosphorylation site of Ser205. AA range:171-220
Specificity
Phospho-PKD1 (S205) Polyclonal Antibody detects endogenous levels of PKD1 protein only when phosphorylated at S205.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from NIH/3T3 cells treated with Anisomycin 25ug/ml 30', using PKD1/PKC mu (Phospho-Ser205) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using PKD1/PKC mu (Phospho-Ser205) Antibody. The picture on the right is blocked with the phospho peptide.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PKD1/PKC mu (Phospho-Ser205) Antibody