The VE-Cadherin (Phospho-Tyr731) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect VE-Cadherin (Phospho-Tyr731) protein expression profile in cells. The kit can be used for measuring the relative amounts of VE-Cadherin (Phospho-Tyr731) in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on VE-Cadherin (Phospho-Tyr731).
This kit contains Crystal Violet Staining normalization protocol. It provides a means to normalize Absorbance 450 nm of primary antibodies to cell density of the cells in the same well. This provides an accurate account for stimulations and cell densities during the assay. By comparing the proportional values obtained from the experimental wells to those of the control wells (e.g. untreated cells), it is possible to determine the extent to which the stimulant(s) have affected the expression levels.
Western Blot analysis of Jurkat cells using VE-Cadherin Polyclonal Antibody diluted at 1:2000. Secondary antibody(catalogNo.:SA0002) was diluted at 1:20000
Western blot analysis of lysates from HepG2 cells treated with Na3VO4 0.3mM 40', using VE-Cadherin (Phospho-Tyr731) Antibody. The lane on the right is blocked with the phospho peptide.