The SLP-76 (Phospho-Tyr128) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect SLP-76 (Phospho-Tyr128) protein expression profile in cells. The kit can be used for measuring the relative amounts of SLP-76 (Phospho-Tyr128) in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on SLP-76 (Phospho-Tyr128).
This kit contains Crystal Violet Staining normalization protocol. It provides a means to normalize Absorbance 450 nm of primary antibodies to cell density of the cells in the same well. This provides an accurate account for stimulations and cell densities during the assay. By comparing the proportional values obtained from the experimental wells to those of the control wells (e.g. untreated cells), it is possible to determine the extent to which the stimulant(s) have affected the expression levels.
Catalog No
CBP2033
Format
Two 96-Well Plates
Detection Target
SLP-76 (Phospho-Tyr128)
Reactivity
Human, Mouse, Rat
Detection Method
Colorimetric 450 nm
Assay Type
Cell-Based ELISA
SwissProt ID
Q13094
NCBI Gene ID
3937
Target Synonyms
LCP2; Lymphocyte cytosolic protein 2; SH2 domain-containing leukocyte protein of 76 kDa; SLP-76 tyrosine phosphoprotein; SLP76
Western blot analysis of lysates from Jurkat cells, treated with EGF 200ng/ml 5', using SLP-76 Antibody. The lane on the right is blocked with the synthesized peptide.