The IGFBP3 (Phospho-Ser183) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect IGFBP3 (Phospho-Ser183) protein expression profile in cells. The kit can be used for measuring the relative amounts of IGFBP3 (Phospho-Ser183) in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on IGFBP3 (Phospho-Ser183).
This kit contains Crystal Violet Staining normalization protocol. It provides a means to normalize Absorbance 450 nm of primary antibodies to cell density of the cells in the same well. This provides an accurate account for stimulations and cell densities during the assay. By comparing the proportional values obtained from the experimental wells to those of the control wells (e.g. untreated cells), it is possible to determine the extent to which the stimulant(s) have affected the expression levels.
Catalog No
CBP2010
Format
Two 96-Well Plates
Detection Target
IGFBP3 (Phospho-Ser183)
Reactivity
Human, Mouse
Detection Method
Colorimetric 450 nm
Assay Type
Cell-Based ELISA
SwissProt ID
P17936
NCBI Gene ID
3486
Target Synonyms
IGFBP3; IBP3; Insulin-like growth factor-binding protein 3; IBP-3; IGF-binding protein 3; IGFBP-3
Western Blot analysis of various cells using IGFBP3 Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).