This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Catalog No
A1052
Reactivity
Human, Mouse
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
IGFBP3; IBP3; Insulin-like growth factor-binding protein 3; IBP-3; IGF-binding protein 3; IGFBP-3
Molecular Weight (Da)
31674
Gene Name
IGFBP3
Protein Name
Insulin-like growth factor-binding protein 3
Human Gene ID
3486
Human Swiss Prot No.
P17936
Immunogen
The antiserum was produced against synthesized peptide derived from human IGFBP-3 around the phosphorylation site of Ser183. AA range:151-200
Specificity
Phospho-IGFBP3 (S183) Polyclonal Antibody detects endogenous levels of IGFBP3 protein only when phosphorylated at S183.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Immunohistochemistry analysis of paraffin-embedded human brain, using IGFBP-3 (Phospho-Ser183) Antibody. The picture on the right is blocked with the phospho peptide.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using IGFBP-3 (Phospho-Ser183) Antibody
