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Akt1 (Phospho-Ser129) Colorimetric Cell-Based ELISA Kit
Akt1 (Phospho-Ser129) Colorimetric Cell-Based ELISA Kit

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: CBP1995
Reactivity:Human,Mouse,Rat
Assay Type:Cell-Based ELISA
Format:Two 96-Well Plates
Range:At least 5000 cells/well
Pairing Option:Non-Phospho or Total Antibody

$500
Product Details
Product Name
Akt1 (Phospho-Ser129) Colorimetric Cell-Based ELISA Kit
Description
The Akt1 (Phospho-Ser129) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect Akt1 (Phospho-Ser129) protein expression profile in cells. The kit can be used for measuring the relative amounts of Akt1 (Phospho-Ser129) in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on Akt1 (Phospho-Ser129). This kit contains Crystal Violet Staining normalization protocol. It provides a means to normalize Absorbance 450 nm of primary antibodies to cell density of the cells in the same well. This provides an accurate account for stimulations and cell densities during the assay. By comparing the proportional values obtained from the experimental wells to those of the control wells (e.g. untreated cells), it is possible to determine the extent to which the stimulant(s) have affected the expression levels.
Catalog No
CBP1995
Format
Two 96-Well Plates
Detection Target
Akt1 (Phospho-Ser129)
Reactivity
Human, Mouse, Rat
Detection Method
Colorimetric 450 nm
Assay Type
Cell-Based ELISA
SwissProt ID
P31749
NCBI Gene ID
207
Target Synonyms
AKT1; PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha
Storage and Stability Guarantee
4°C/6 Months
Sample Type
Researchers plate their cell line of choice
Range
At least 5000 cells/well
Time
4.5 hours
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