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Akt (Phospho-Ser473) Colorimetric Cell-Based ELISA Kit

Catalog No : CBP1994
  • Reactivity
    Human,Mouse,Rat
  • Assay Type
    Cell-Based ELISA
  • Format
    Two 96-Well Plates
  • Range
    At least 5000 cells/well
  • Pairing Option
    Non-Phospho or Total Antibody
  • Size
    1 kit
  • Price/Quantity
    $500
  • Quantity
Details
  • Product Name
    Akt (Phospho-Ser473) Colorimetric Cell-Based ELISA Kit
  • Description
    The Akt (Phospho-Ser473) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect Akt (Phospho-Ser473) protein expression profile in cells. The kit can be used for measuring the relative amounts of Akt (Phospho-Ser473) in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on Akt (Phospho-Ser473). This kit contains Crystal Violet Staining normalization protocol. It provides a means to normalize Absorbance 450 nm of primary antibodies to cell density of the cells in the same well. This provides an accurate account for stimulations and cell densities during the assay. By comparing the proportional values obtained from the experimental wells to those of the control wells (e.g. untreated cells), it is possible to determine the extent to which the stimulant(s) have affected the expression levels.
  • Catalog No
    CBP1994
  • Format
    Two 96-Well Plates
  • Detection Target
    Akt (Phospho-Ser473)
  • Reactivity
    Human, Mouse, Rat
  • Detection Method
    Colorimetric 450 nm
  • Assay Type
    Cell-Based ELISA
  • SwissProt ID
    P31749
  • NCBI Gene ID
    207
  • Target Synonyms
    AKT1; PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha
  • Storage and Stability Guarantee
    4°C/6 Months
  • Sample Type
    Researchers plate their cell line of choice
  • Range
    At least 5000 cells/well
  • Time
    4.5 hours